In vitro primary hepatocyte assay

With the rise of metabolism related disorders such as obesity, diabetes, and other metabolic diseases, in vitro hepatic cell and tissue models play an essential role in the identification of active pharmaceutical ingredients.

Compared with hepatic cell line, primary hepatocytes indicate stronger glucose metabolic abilities in terms of glucose consumption, intracellular glycogen content, gluconeogenesis rate, and sensitivity to glucose modulator hormones like insulin and glucagon.

MB Bioscience offer in vitro platforms based on primary hepatocytes and hepatic cells for defining glucose and lipid metabolism profiles and screening therapeutic agents for metabolism disorders like diabetes and obesity. As a leading biotechnology company in hepatic cells, MB bioscience provide a wide range of tests using primary human, rat, and mouse hepatocytes: 

• Urea secretion

• Albumin secretion

• Hepatic glycogen synthesis

• Hepatic glucose production

• Glycogenolysis

• Gluconeogenesis

• lipolysis assays

• lipids and phospholipids accumulation

• lipid peroxidation

• LDL uptake

• NADH/FAD content

• ATP content

• LDH release

• intracellular ROS

• intracellular Glutathione (GSH) content

• lipogenesis

• Lactate output

• Hepatic Lipid Accumulation

• Extracellular acidification rate 

• Hepatocyte cAMP production

• Hepatocyte oxygen consumption

• Liver specific gene expression analysis

• Steatosis 

• Cholestasis

• Phospholipidosis

• chronic DILI and acute DILI

• Glucokinase activation

• Pyruvate carboxylase inhibition

• EROD assay

• Cytochrome-P450 Induction

• Oxidative Stres