HEPSPANLONG-TERM CULTURE PRIMARY HEPATOCYTE MEDIUM AND HEPSPANLONG-TERM CULTURE PRIMARY HEPATOCYTE

HEPSPANLONG-TERM CULTURE PRIMARY HEPATOCYTE MEDIUM

Current standard monolayer hepatocyte cultures are typically functional in culture for 5 to 7 days. There is an increasing need for longer term cultures of hepatocytes to study liver toxicity, drug metabolism, drug interactions, viral infection, inflammation, liver development and regeneration. In order to extend the functional life of hepatocytes in culture, we have developed HepSpan™ medium, a long-term culture of primary hepatocyte medium which maintains the morphology and function of primary hepatocyte in standard 2D cultures for 4 weeks (human hepatocytes), 3 weeks (rat hepatocytes) and 2 weeks (mouse hepatocytes).

HepSpan™ medium is optimized for primary hepatocyte to enhance cell viability, function and long-term maintenance. HepSpan™ is a better representation of the liver’s extracellular environment for the initiation and maintenance of primary hepatocytes long-term cultures.

sGdVl-GO0uaGyNtVG5lScFA.png
saaVpi4lXNMpY4CU-Pf0fxw.png

HepSpan Medium Advantages

  • Better representation of the liver’s extracellular environment

  • Improved hepatocytes function and high levels of liver-specific gene expression

  • Stable albumin and urea secretion for four weeks in culture

  • Maintain Long-term CYP activities and CYP inducibility of primary hepatocytes

  • Support long-term culture of cryopreserved and fresh plateable primary hepatocytes 

HepSpanMedium Features

  • Defined medium tailored to support hepatocyte long term in vitro culture 

  • Adjusted to standard 2D monolayer culture and high throughput

  • Raw material screening and quality control ensure minimal lot-to-lot variability

  • Multi-species culture medium available (Human, Rat, Mouse, Dog, Monkey)

HepSpanMedium Application

  • Hepatic Disease Model

  • Drug Metabolism

  • Metabolic Disorders Study

  • Drug induced liver injury (DILI) Study

  • Drug Transporters and Drug-Drug Interaction

Assessment of 4 weeks cultured Hepatic Functions of Human Hepatocytes utilizing HepSpan™ Media

Assessment of 4 weeks cultured Hepatic Functions of HepSpan™ primary human hepatocytes
Albumin secretion (A), Urea secretion (B), Glucose output (C), cAMP production (D), CYP3A4 enzyme activity (E), and CYP1A2 enzyme activity (F)

Albumin secretion (A), Urea secretion (B), Glucose output (C), cAMP production (D), CYP3A4 enzyme activity (E), and CYP1A2 enzyme activity (F)

HepSpan™ Maintenance Medium

ProductCatalog NumberList Price
Human HepSpan™ Hepatocyte Maintenance MediumHM-8002$449
Rat HepSpan™ Hepatocyte Maintenance MediumRM-8002$499
Mouse HepSpan™ Hepatocyte Maintenance MediumMM-8002$499
Dog HepSpan™ Hepatocyte Maintenance MediumDM-8002$499
Monkey HepSpan™ Hepatocyte Maintenance MediumCM-8002$499

HepSpanReady-to-Use Long-Term Cultured Primary Hepatocyte

Primary hepatocytes and their adherent cultures are the gold-standard in vitro tool to evaluate liver toxicity, drug metabolism, cytochrome P450 induction, drug-drug interactions, viral infection, inflammation, liver development and regeneration.  However, primary hepatocytes rapidly lose their morphology and liver-specific functions, such as detoxification, activity of phase I and phase II enzymes, and the production of plasma proteins like albumin over a few days in standard culture. 

MB Biosciences also provide HepSpan™ Ready-to-use long term cultured primary hepatocytes which include a pre-seeded hepatocytes in multi-well formats plates with long-term culture medium to overcome the short stability culture problem as well as standard issues of working with primary hepatocyte such as hepatocyte plating failure, poor viability, and alleviates any unnecessary time and cost spent on finding exceptional lots.

HepSpan™ ready-to-use cultured primary hepatocytes with a prolonged culture up to 4 weeks for human hepatocytes, 3 weeks for rat hepatocytes and 2 weeks for mouse hepatocytes in standard 2D-culture formats (24 wells, 48 wells, 96 wells and 384 wells).

HepSpan™ ready-to-Use Hepatocytes is an innovative platform for ADME/TOX, viral infection, inflammation and liver biology study. They are stored and transported at room temperature, and enables the ability to perform the experiments that require a longer time culture of primary hepatocytes.

HepSpan™ Ready-to-Use Long-Term Cultured Primary Hepatocyte Features

  • Ready-To-Use for your assay right after delivery

  • High quality pre-screened human primary hepatocytes and fresh animal hepatocytes

  • Full Hepatocytes functionality after transportation

  • Maintain Long-term CYP activities and CYP inducibility of primary hepatocytes

  • Stable albumin and urea secretion for four weeks in culture

  • Don't need Matrigel overlays, complicated 3D culture systems or bioreactors

HepSpan™ Ready-to-Use Long-Term Cultured Primary Hepatocyte Benefits

  • Time saving and cost-effective

  • Not employ genetically modified human hepatocyte cells or cell lines.

  • High levels of liver-specific gene expression, metabolic and synthetic function 

  • Easily adaptable to automated procedures and HTS 

  • Multi-species available (Human, Rat, Mouse, Dog, Monkey)

ProductCatalog NumberList Price
HepSpan™ Human Hepatocytes, 6-well formatHS-06Inquiry
HepSpan™ Human Hepatocytes, 12-well formatHS-12Inquiry
HepSpan™ Human Hepatocytes, 24-well formatHS-24Inquiry
HepSpan™ Human Hepatocytes, 48-well formatHS-48Inquiry
HepSpan™ Human Hepatocytes, 96-well formatHS-96Inquiry
HepSpan™ Human Hepatocytes, 384-well formatHS-384Inquiry
HepSpan™ Rat Hepatocytes, 6-well formatRS-06$750
HepSpan™ Rat Hepatocytes, 12-well formatRS-12$750
HepSpan™ Rat Hepatocytes, 24-well formatRS-24$750
HepSpan™ Rat Hepatocytes, 48-well formatRS-48$750
HepSpan™ Rat Hepatocytes, 96-well formatRS-96$750
HepSpan™ Rat Hepatocytes, 384-well formatRS-384$850
HepSpan™ Mouse Hepatocytes, 6-well formatMS-06$950
HepSpan™ Mouse Hepatocytes, 12-well formatMS-12$950
HepSpan™ Mouse Hepatocytes, 24-well formatMS-24$950
HepSpan™ Mouse Hepatocytes, 48-well formatMS-48$950
HepSpan™ Mouse Hepatocytes, 96-well formatMS-96$950
HepSpan™ Mouse Hepatocytes, 384-well formatMS-384$1,050
HepSpan™ Dog Hepatocytes, 96-well formatDS-96Inquiry
HepSpan™ Dog Hepatocytes, 384-well formatDS-384Inquiry
HepSpan™ Monkey Hepatocytes, 96-well formatCS-96Inquiry
HepSpan™ Monkey Hepatocytes, 384-well formatCS-384Inquiry