Primary Hepatocyte isolation for Gene Therapy & Gene Editing 

Disease-relevant hepatocyte models. Expertly isolated. Delivered ready for transduction and editing.

Accelerate gene therapy development — we handle the hepatocytes

Primary hepatocytes are the most biologically relevant in vitro system for evaluating gene delivery, expression, editing efficiency, and functional rescue. However, isolating and maintaining high-quality hepatocytes from normal and diseased livers is technically complex and time-consuming.

We provide a turnkey solution.
From isolation through stabilized culture, we deliver ready-to-use 2D and 3D primary hepatocyte models, enabling your team to focus on vector design, gene correction, and functional validation.

Expert hepatocyte isolation for genetically relevant models

  • Extensive experience isolating rat and mouse primary hepatocytes

  • Routine work with genetic and metabolic liver disease models

  • Specialized expertise in maintaining hepatocyte differentiation and function

  • Reliable delivery of 2D monolayer and 3D spheroid hepatocyte systems

Isolation optimized for diseased and genetically altered livers

Standard liver perfusion methods often fail in diseased or genetically modified livers, resulting in:

  • Selective loss of fragile hepatocytes

  • Poor recovery of lipid-laden or stressed cells

  • Underrepresentation of disease-relevant cell populations

Our proprietary, modified liver perfusion and gradient centrifugation protocol is specifically designed to:

  • Preserve lipid-rich and disease-relevant hepatocytes

  • Improve yield and viability from steatotic, fibrotic, and genetically altered livers

  • Maintain phenotypes critical for gene therapy evaluation

Proven experience with gene therapy–relevant disease models

We have successfully isolated and cultured hepatocytes from:

  • Non-alcoholic fatty liver disease (NAFLD / MAFLD)
    (rat and mouse models)

  • PiZ transgenic mouse (α1-antitrypsin deficiency)

  • Gunn rat (bilirubin metabolism disorder)

  • Ldlr⁻/⁻ mouse and rat (familial hypercholesterolemia)

  • Humanized mouse models

  • Gene knockout and transgenic mouse models

Ready-to-use hepatocytes for gene therapy workflows

Hepatocyte isolation

  • Model-specific perfusion and enzymatic digestion

  • Optimized recovery of fragile and disease-relevant hepatocytes

  • Rigorous quality control to ensure consistency

Gene therapy–compatible hepatocyte culture

  • 2D hepatocyte cultures optimized for:

    • AAV, LNP, and viral vector transduction

    • CRISPR/Cas and base-editing workflows

  • 3D hepatocyte spheroids supporting:

    • Long-term gene expression and durability studies

    • Functional rescue and chronic disease modeling

  • Hepatocytes delivered plated, stabilized, assay-ready, or cryopreserved

2D and 3D formats to support gene therapy development

2D hepatocyte cultures

Ideal for:

  • Vector screening and tropism studies

  • Transduction efficiency and dose–response

  • Gene-editing efficiency and on-/off-target assessment

  • Short-term expression and mechanistic studies

3D hepatocyte spheroids

  • Sustained liver-specific function over extended culture

  • Suitable for long-term expression, durability, and repeat-dosing studies

  • Enhanced physiological relevance for translational decision-making

Why gene therapy teams choose us

✔ Deep experience with genetic and metabolic liver disease models
✔ Isolation protocols preserving true disease-relevant hepatocytes
✔ Cultures compatible with viral vectors and gene-editing technologies
✔ End-to-end service reducing internal technical burden
✔ Consistent, high-quality hepatocytes ready for gene therapy workflows