Why Choose Our HepSpan™ Ready-To-Use 3D Primary Hepatocyte Spheroid and Liver Organoid?

MB Biosciences offers HepSpan™ ready-to-use 3D primary hepatocyte spheroids and liver organoids designed to advance liver research and drug discovery. These physiologically relevant 3D cultures closely mimic native liver tissue architecture, providing a more predictive and effective platform for studying liver function, toxicity, drug metabolism, viral infection, inflammation, and liver development and regeneration.

Our primary hepatocyte spheroids are generated using ultra-low attachment culture systems to promote consistent 3D formation. Available in 24-,  96-, and 384-well plate formats, HepSpan™ spheroids and organoids are delivered ready for immediate use and maintain stable expression of key functional markers for at least 30 days.

MB Biosciences also offers scalable production of primary human and animal hepatocyte spheroids with tightly controlled size and high uniformity, ensuring reproducibility across experiments and compatibility with high-throughput applications. Our platform is designed to deliver superior physiological relevance, flexibility, and ease of use—addressing key limitations of conventional 3D liver models on the market.

Key Advantages

1. Ready-to-Use Convenience
Our spheroids and organoids are delivered ready for immediate use, eliminating time-consuming setup and reducing variability associated with in-house model generation. Simply receive, equilibrate, and start your assays.

2. Proprietary Shipping Medium for Protecting 3D structure and Preserving Viability
We utilize a proprietary shipping medium that preserves spheroid and organoid integrity during transit by:

• Maintaining cell viability and functionality 

• Protecting 3D structure stability 

• Minimizing shipping-induced stress 

This ensures consistent, high-quality performance upon arrival.

3. Freshly Isolated Primary Hepatocytes
We use freshly isolated mouse and rat primary hepatic cells to generate spheroids and organoids, ensuring higher viability, preserved metabolic function, and more in vivo–like responses compared to cryopreserved or passaged cells.

4. Consistent, Uniform 3D Structures
Each spheroid and organoid is pre-formatted for uniform size and cell number, enabling:

• Reduced well-to-well variability 

• Improved reproducibility 

• More reliable quantitative data 

5. Defined Cell Number Per Spheroid
Unlike many suppliers, we use the same number of primary hepatocytes per spheroid/organoid, allowing for:

• Better experimental control 

• Easier data normalization 

• Enhanced comparability across studies 

6. Flexible Plate Formats for Scalable Workflows
Available in 24-, 96-, and 384-well formats, supporting both:

• High-throughput screening 

• Mechanistic and low-throughput studies 

7. Single or Multi-Spheroid Per Well Options
We offer both one spheroid per well and multiple spheroids per well, providing critical flexibility:

• Single spheroid per well
  Ideal for imaging, secretion assays, and longitudinal studies 

• Multiple spheroids per well
  Enables sufficient biomass for more demanding downstream assays, such as: 

• Western blot 

• Enzyme activity assays 

• Protein/RNA extraction 

8. Compatible with Standard Lab Equipment
Our product formats are designed for seamless integration into existing workflows and are fully compatible with standard laboratory equipment, including:

• Plate readers 

• Imaging systems 

• Automated liquid handling platforms 

9. Overcoming Industry Limitations
Most commercially available systems provide only one spheroid per well, which can be limiting for assays requiring higher material input.
Our multi-spheroid format eliminates the need for sample pooling and improves data consistency.

Applications

Our 3D hepatocyte spheroids and liver organoids are ideal for:

• Drug-induced liver injury (DILI) assessment 

• Hepatotoxicity screening 

• Metabolism and clearance studies 

• Transporter function and cholestasis assays 

• Long-term and chronic toxicity studies

• Gene editing and mRNA delivery

Our 3D primary hepatocyte spheroids and liver organoids are engineered to overcome the limitations of traditional single-spheroid and complex microphysiological systems with ready-to-use, scalable 3D liver models designed for real-world assays.

HepSpan™ Primary Hepatocyte Spheroid

HepSpan™ Primary Liver Organoid

Primary hepatocyte, Kupffer cell, and liver endothelial cell (LEC) co-cultures